Coding

Part:BBa_K1531004:Design

Designed by: Patrik D   Group: iGEM14_SF_Bay_Area_DIYbio   (2014-10-13)


Bovine beta casein (B variant) with full alpha-factor yeast secretion signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1
    Illegal SapI.rc site found at 910


Design Notes

The DNA sequence was codon optimized for expression in S. cerevisiae, using IDT's codon optimization tools, followed by manual editing of a few codons to avoid specific restriction sites.


Source

Bos taurus sequence, from http://www.ncbi.nlm.nih.gov/protein/AAI11173.1

References